TRANSFOG
TRANSlational and Functional Onco-Genomics
Sixth_Framework
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Partner 4: DKFZ
Partner name: Deutsches Krebsforschungszentrum
Short name: DKFZ
Country: Germany
Role: CR (contractor)
The DKFZ has carried out systematic screens with expression profiling in a number of cancers, including renal clear cell carcinoma, breast cancer, brain tumors, and GIST. In previous work within the German cDNA Consortium the partner has identified and cloned in a GATEWAY-compatible manner a set of 800 full-length open reading frames coding for proteins with unknown function. Of these 400 have already been pre-characterized with regard to the subcellular localization of the encoded proteins and 500 have been cloned for native protein expression. High-throughput assays for cell proliferation (bromodeoxyuridine incorporation) and apoptosis (Caspase-3 cleavage) are already operative. Databases for management and integration of information with data obtained from expression profiling and protein arrays have been set up. Tools for the web-presentation of this data are under development (www.dkfz.de/LIFEdb). Given its expertise, the DKFZ will coordinate the workpackage 2, which is aimed at producing and delivering full-length verified ORFs.
PERSONNEL :
Name
Title
Sex
Relevant Expertise
Annemarie Poustka PROF
F
Head of Division
Peter Lichter PROF
M
Head of Division
Holger Sültmann DR
M
RNA expression profiling
Stefan Wiemann DR
M
cDNA based technologies
Dorit Arlt DR
F
cell-based assays
Lee Bergman DR
F
cell-based assays
Stefanie Bechtel DR
F
cDNA resources
Boris Zielinski DR
M
CGH and matrix CGH
REFERENCES:
  • Wiemann et al., 2001. Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs. Genome Res 11, 422-435.
  • Wessendorf et al., 2002. Automated screening for genomic imbalances using matrix-based comparative genomic hybridization. Lab Invest 82(1):47-60
  • Mollenhauer et al., 1997. DMBT1, a new member of the SRCR superfamily, on chromosome 10q25.3-q26.1 is deleted in malignant brain tumours. Nature Genet. 17, 32-39 (1997).
  • Fritz et al., 2002. Microarray-based copy number and expression profiling in dedifferentiated and pleomorphic liposarcoma. Cancer Res 2002 Jun 1;62(11):2993-8
  • Simpson, J. C., Wellenreuther, R., Poustka, A., Pepperkok, R., and Wiemann, S. Systematic subcellular localization of novel proteins identified by large scale cDNA sequencing. EMBO Rep 1, 287-292 (2000).
UPCOMING EVENT
OECI week

OECI Week (WG workshops; Scientific Conference and General Assembly) – Budapest, 16 to 18 June 2010.
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